Biosensor construction
Gibson assembly
All cloning, except for site-directed mutagenesis, was conducted using Gibson assembly (NEBuilder HiFi DNA Assembly Master Mix, New England Biolabs)54. All pr… [+31252 chars]
Ligand-specific activation trajectories dictate GPCR signalling in cells - Nature
Different agonists produce equilibria of at least four distinct active states of the G-protein-bound M2 muscarinic acetylcholine receptor, each with a different ability to activate G proteins.
Source:Nature.com
Published:
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